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. 2014 Dec 11;21(5-6):970–981. doi: 10.1089/ten.tea.2013.0789

FIG. 3.

FIG. 3.

The alamarBlue™ fluorescence and alkaline phosphatase (ALP) activity of mMSCs seeded onto the various scaffolds. Murine MSCs were seeded onto the various scaffolds in either maintenance medium (Iscove's modified Dulbecco's medium) or osteogenic medium (OGM). AB fluorescence (for metabolic activity) and ALP activity (for osteogenic differentiation) were measured at the times indicated (for details see Materials and Methods section). All data are presented as means±standard deviations. Sample size was n=3 (in triplicate) with *p<0.05 and **p<0.01 compared with the previous time points of the same group and ++p<0.01 compared with the CTS-GP control group at the same time point. (A) Continual measurement of alamarBlue™ fluorescence revealed that by day 10 the metabolic activity of mMSCs decreased on all scaffolds, and plateaued until day 21 (data are expressed as arbitrary fluorescence units). (B) ALP activity normalized to mMSCs cultured in parallel on tissue culture polystyrene (TCP), increased in the presence of HA and OGM when compared with CTS-GP cultures alone.