FIG. 3.

In vitro viability and apoptosis of cultured HSs, ICCs, and hMSC spheroids. (a) Long-term in vitro viability of ICCs, HSs, and hMSC spheroids on day 7 as evaluated by live/dead assay. Scale bars=100 μm. (b) Live/dead images (left panel) and their relative quantification graph (right panel) of ICCs, HSs (10:1), and hMSC spheroids cultured under normoxic and hypoxic conditions for 2 days. Green and red signals indicate live and dead cells, respectively. Scale bars=100 μm. *p<0.05 compared with ICCs. (c) Relative expression of Bax and Bcl-2 of ICs in ICCs or HSs cultured under normoxic or hypoxic condition, as evaluated by qRT-PCR. *p<0.05 compared with ICCs. qRT-PCR, quantitative reverse transcriptase-polymerase chain reaction. Color images available online at www.liebertpub.com/tea