FIG. 4.

Analysis of angiogenic factors present in cell culture supernate, and human HIF-1α mRNA expressions in monolayer hMSCs and hMSCs of HSs on day 3. (a) The relative amounts of human angiogenic factors (TIMP-1, IGFBP-3, CXCL8, and VEGF) present in cell culture supernate of HSs (10:1). Cell culture supernate was collected on days 3 and 5 and subjected to a human angiogenesis antibody array. Since the data represent the average values from a single test, which yielded two test values for each individual cytokine, statistical significance was not determined. (b) The amounts of hVEGF secreted from rat ICCs and HSs (10:1) containing hMSCs and rat ICs cultured in normoxic conditions and HSs (10:1) cultured in hypoxic conditions at various culture time points, as evaluated by ELISA. The red dashed line indicates 0 pg/ml of hVEGF. p<0.05 between any two groups at all time points. (c) Human HIF-1α mRNA expressions of monolayer hMSCs or HSs on day 3. CXCL8, interleukin-8; ELISA, enzyme-linked immunosorbent assay; HIF-1α, hypoxia inducible factor-1α; hVEGF, human vascular endothelial growth factor; IGFBP-3, insulin-like growth factor-binding protein-3; TIMP-1, tissue inhibitor of metalloproteinase-1; VEGF, vascular endothelial growth factor. Color images available online at www.liebertpub.com/tea