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Cell labeling and co-staining of collagen type II in mixed co-culture. To identify the relative contribution of each cell type to collagen type II deposition in mixed co-culture, OACs (red) and ADSCs (green) were fluorescently labeled before encapsulation in hydrogels and co-stained with collagen type II (blue) after 21 days of mixed co-culture without (A) or with (B) TGF-β3 supplementation. Scale bar=100 μm. Color images available online at www.liebertpub.com/tea
