FIG. 2.

TEE demonstrates epithelial differentiation and proliferation. (A–D) Native esophagus. (E–H) TEE harvested at 4 weeks. Positive staining for both cytokeratin 4 (A, E) and cytokeratin 13 (B, F) confirms the presence of differentiated suprabasal cells. Positive cytoplasmic staining for cytokeratin 14 (C, G) and positive nuclear staining for proliferating cell nuclear antigen (PCNA) (D, H) confirms the presence of proliferative basal cells. DAPI stains all nuclei blue. Scale bars=50 μm. Color images available online at www.liebertpub.com/tea