FIG. 5.
TEE demonstrates variable donor origin of epithelium and mesenchyme. (A–D) TEE was generated from pooled organoid units of actinGFP, B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J, and wild-type donor mice. TEE lumen is oriented toward the top left corner of each image. Immunofluorescence costaining for green fluorescent protein (GFP) and red fluorescent protein (RFP) demonstrates conservation of original esophageal organoid unit (EOU) epithelial–mesenchymal relationships in some samples (A, B) where the epithelial and mesenchymal layers are of the same origin. Both the epithelial and mesenchymal layers in (A) stain positive for GFP, and in (B) both layers stain positive for RFP. However, other TEE samples (C, D) show variable donor origin of epithelium and mesenchyme. The TEE epithelium in (C) stains positive for RFP while its subjacent mesenchyme is GFP+. (D) Shows TEE with even greater heterogeneity of donor origin with an epithelial layer that is GFP+ on the left but RFP+ on the right and a mesenchyme of predominantly GPF+ with occasional RFP+ cells. DAPI stains all nuclei blue. Scale bars=50 μm. Color images available online at www.liebertpub.com/tea