FIG. 2.

Workflow for sample processing, fractionation, and proteomic analysis of L. donovani glycosomes. L. donovani promastigotes were used for the isolation and purification of glycosomes for proteomic analysis as indicated. Proteins from the glycosome were extracted using SDS and subjected to SDS-PAGE or digested with trypsin and subjected to strong cation exchange (SCX) chromatography. LC-MS/MS analysis of the digested gel bands or SCX fractions was carried out on a high resolution mass spectrometer. The mass spectrometry data were analyzed against a protein database of L. donovani.