FIG. 3.
Identification of PTS-1 containing protein ATP-dependent phosphofructokinase. (A) ATP-dependent phosphofructokinase (blue bar) in L. donovani is encoded by the gene LDBPK_292620 encoding a 486 amino acid long protein. The red bars indicate the peptides identified in our study mapping to ATP-dependent phosphofructokinase. (B) Sequence of ATP-dependent phosphofructokinase (LDBPK_292620) with its C-terminal PTS-1 sequence “SKV” marked in gray, and the 21 unique peptides that were identified marked in red. (C) A representative MS/MS spectrum of peptide TFGFQTAVEQAVNAVR identified from ATP-dependent phosphofructokinase.