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| Method | Sitting-drop vapour diffusion |
| Plate type | MRC 96-well plate |
| Temperature (K) | 277 |
| Protein concentration (mgml1) | 13.5 |
| Buffer composition of protein solution | 20mM TrisHCl pH 8.0, 300mM NaCl, 10mM Na2HPO4, 5mM dithiothreitol |
| Composition of reservoir solution | 100mM Na HEPES pH 7.0, 15%(v/v) polyethylene glycol 20000 |
| Volume (l) and ratio of drop | 2 (1:1 ratio of protein and reservoir) |
| Volume of reservoir (l) | 70 |
