Table 1. Macromolecule cloning and expression conditions.

Source organism	S. thermophila
DNA source	Synthetic DNA
Cloning vector	pBlueScript
Expression vector	pET-28a
Expression host	E. coli SlyD pRARE
Complete amino-acid sequence of the construct†	mgsshhhhhhssglvprgshmSTPGGGEYTEIALPFSYDGAGEYYWKTDDFSTTTNWGRYVNSWNLDLLEINGTDYANTWVPQHAIPPASDGYWYIHYKGSYPWSHVEM

^†The histidine tag from the vector is represented in lower case in the amino-acid sequence. The tag was removed for the crystallization experiments.