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. 2015 Feb 19;71(Pt 3):315–319. doi: 10.1107/S2053230X15002290

Table 2. Crystallization.

Method Vapour diffusion
Plate type Sitting-drop MRC 96-well
Temperature (K) 295
Protein concentration (mgml1) 16.5
Buffer composition of protein solution 20mM Tris pH 7.0, 200mM NaCl, 5mM MgCl2, 5mM -mercaptoethanol, 10% glycerol
Composition of reservoir solution 0.1M TrisHCl pH 8.5, 0.2M MgCl2, 0.5M NaCl, 32.5% PEG 3350