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The BamHI and HindIII restriction sites are underlined.
| Isoform | 5 | 9 |
|---|---|---|
| Source organism | D. melanogaster | D. melanogaster |
| DNA source | cDNA | cDNA |
| Forward primer | 5-CGGGATCCGTTCCACCGTCGATAGC-3 | 5-CGGGATCCGTGCCGCCCCAGGTTT-3 |
| Reverse primer | 5-CCCAAGCTTTCAGTTGACTTTCAGCTCCGA-3 | 5-CCCAAGCTTTCAATTAACTTGTAATTCA-3 |
| Expression vector | pET-28a(+) | pET-28a(+) |
| Expression host | E. coli strain Rosetta(DE3) | E. coli strain SHuffle |
