| Method |
Hanging-drop vapour diffusion |
Hanging-drop vapour diffusion |
| Plate type |
24-well |
24-well |
| Temperature (K) |
289 |
289 |
| Protein concentration (mgml1) |
15 |
15 |
| Buffer composition of protein solution |
20mM HEPES pH 7.5, 100mM NaCl |
20mM HEPES pH 7.5, 100mM NaCl |
| Composition of reservoir solution |
0.1M bis-tris pH 6.5, 50mM calcium chloride, 30%(w/v) polyethylene glycol monomethyl ether 550 |
0.1M HEPES pH 7.5, 0.7M sodium phosphate monobasic, 0.7M potassium phosphate monobasic |
| Volume and ratio of drop |
1:1 |
1:1 |
| Volume of reservoir (l) |
500 |
500 |
