Table 2. Specific activity of HoAraf43 on pNP-Araf.
Specific activity† (molmin1mg1) | ||
---|---|---|
Reaction conditions | Before adding Na2CO3 | After adding Na2CO3 |
4M NaCl, 2mM Mg2+, EDTA/EGTA-treated HoAraf43 | 6.68 0.32 | 17.38 0.20 |
4M NaCl, 2mM Mg2+, untreated HoAraf43 | 6.69 1.2 | 15.86 0.56 |
4M NaCl, 2mM Mn2+, EDTA/EGTA-treated HoAraf43 | 20.32 0.49 | 36.20 5.6 |
4M NaCl, 2mM Mn2+, untreated HoAraf43 | 20.78 1.7 | 22.89 7.0 |
Expressed as the arithmetic mean and the standard error of the mean.