Table 1. Summary of the constructs used in this study.

tCid1 RNA-binding mutant
Primer nucleotide sequences
K133A/R137A-F	aggatttgaaggaGCatttttacaaGCggcaagaattccca
K133A/R137A-R	tgggaattcttgccGCttgtaaaaatGCtccttcaaatcct
R277A/K282A-F	cttcatggcttttttGCattttatgcttatGCgttcgagccacg
R277A/K282A-R	cgtggctcgaacGCataagcataaaatGCaaaaaagccatgaag
Amino-acid sequence of resulting protein†	GPLGSSYQKVPNSHKEFTKFCYEVYNEIKISDKEFKEKRAALDTLRLCLKRISPDAELVAFGSLESGLALKNSDMDLCVLMDSRVQSDTIALQFYEELIAEGFEGAFLQAARIPIIKLTSDTKNGFGASFQCDIGFNNRLAIHNTLLLSSYTKLDARLKPMVLLVKHWAKRKQINSPYFGTLSSYGYVLMVLYYLIHVIKPPVFPNLLLSPLKQEKIVDGFDVGFDDKLEDIPPSQNYSSLGSLLHGFFAFYAYAFEPREKVVTFRRPDGYLTKQEKGWTSATEHTGSADQIIKDRYILAIEDPFEISHNVGRTVSSSGLYRIRGEFMAASRLLNSRSYPIPYDSLFEEAPIPPRRQKKTDEQSNKKLLNETDGDNSE
Minimal Cid1 (mCid1)
Primer nucleotide sequences
Forward	aagttctgtttcagggcccgCACAAGGAATTTACGAAGTTTTGC
Reverse	atggtctagaaagctttaGGCCTCCTCAAATAATGAATCATAA
Amino-acid sequence of resulting protein†	MAHHHHHHSSGLEVLFQGPHKEFTKFCYEVYNEIKISDKEFKEKRAALDTLRLCLKRISPDAELVAFGSLESGLALKNSDMDLCVLMDSRVQSDTIALQFYEELIAEGFEGKFLQRARIPIIKLTSDTKNGFGASFQCDIGFNNRLAIHNTLLLSSYTKLDARLKPMVLLVKHWAKRKQINSPYFGTLSSYGYVLMVLYYLIHVIKPPVFPNLLLSPLKQEKIVDGFDVGFDDKLEDIPPSQNYSSLGSLLHGFFRFYAYKFEPREKVVTFRRPDGYLTKQEKGWTSATEHTGSADQIIKDRYILAIEDPFEISHNVGRTVSSSGLYRIRGEFMAASRLLNSRSYPIPYDSLFEEA

^†Protein sequences are of the protein used for crystallization.