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. 2015 Feb 28;71(Pt 3):354–357. doi: 10.1107/S2053230X15001028

Table 2. Crystallization conditions for the crystal that was used for diffraction data collection.

Method Hanging-drop vapour diffusion
Plate type 24-well plates
Temperature (K) 289
Protein concentration (mgml1) 3.5
Buffer composition of protein solution 50mM TrisHCl pH 7.5, 150mM NaCl
Composition of reservoir solution 0.05M sodium cacodylate pH 6.5, 0.2M KCl, 2.5%(w/v) hexanediol
Volume and ratio of drop 2l drop: 1l protein solution and 1l reservoir solution
Volume of reservoir (l) 200