Table 1.
Information on previously published and newly developed microsatellite primers for Sequoia sempervirens.
| Locus (label) | GenBank accession no. | Primer sequences (5′–3′) | No. of PCR extension cycles | Ta (°C) | Allele size range (bp) | Aa | Hoa | No. of individuals with 1/2/3/4/5/6 alleles detecteda |
| RW28 (FAM) | GU969047 | F: GATAGATAAATAGATGGATAG | 35 | 65–50 | 187–342 | 19 | 0.39 | 219/106/29/5/0/0 |
| R: TTTTTAAGGTTTCATGGATAAGTACAA | ||||||||
| RW39 (FAM) | GU969046 | F: CCATAAGGTTGAAATGAAGAAAAA | 30 | 65–50 | 240–470 | 69 | 1.00 | 2/9/52/138/149/97 |
| R: GTTGATTGATCGTTGGTTGG | ||||||||
| SEQ18D7-3 (HEX) | AY562168 | F: GCAAAAAGGGAATTGTAATTGGGTTCA | 27 | 67–52 | 124–183 | 13 | 0.68 | 136/180/88/15/5/0 |
| R: CCCTAGGTCTAGGCTACGCGACTTG | ||||||||
| SEQ8E8 (FAM) | AY562169 | F: ATACTCACCCTTACACGGGC | 28 | 67–52 | 112–185 | 21 | 0.24 | 246/65/9/2/0/0 |
| R: AAATGCCTTGATGAAGCAAAA | ||||||||
| RW56 (HEX) | KP055095 | F: CTTGACATCATCCATAGCT | 30 | 69–54 | 189–259 | 18 | 0.89 | 51/116/166/89/23/1 |
| R: AAATTGCAAGGGGTGCAA | ||||||||
| RWDI11 (HEX) | KP055096 | F: GGACCAAATGCCCTGAAC | 30 | 63–48 | 215–268 | 29 | 1.0 | 1/19/65/174/142/48 |
| R: GCCAAGCCATATGGGTTTG |
Note: A = number of alleles; Ho = observed heterozygosity; Ta = annealing temperature.
a
A, Ho, and numbers of individuals with each possible allele count were calculated using combined data from all six study plots.