Table 1.
Characteristics of 11 microsatellite loci developed in Phytolacca americana.
| Locus | Primer sequences (5′–3′) | Repeat motif | Allele size range (bp)a | Ta (°C) | Fluorescent dyeb | GenBank accession no. |
| PW11 | F: AGCCGGAGGTCTCTCTTGG | (AAC)33 | 354–372 | 65–55 | FAM | KP331491 |
| R: *CAGTTTAGAAATCTGGAATTAGAGTTGG | ||||||
| PW29 | F: *GATGAAGAAAGGGCAACCCC | (AATAAG)30 | 354–366 | 65–55 | HEX | KP331492 |
| R: ACGAGTGCAGATCCAAGTGC | ||||||
| PW46 | F: *GGATGCAAATAATCCTAGTTCGG | (ATT)36 | 331–352 | 65–55 | HEX | KP331493 |
| R: CAGACTCCCGAGTTTGTCCC | ||||||
| PW53 | F: AATCTTTGGCTTGGCCACC | (ATAC)24 | 454–458 | 65–55 | HEX | KP331494 |
| R: *TCAAAAGACAATGCAGAAGCC | ||||||
| PW54 | F: *CATATCACGAGAGGGGAGCC | (AAAAG)30 | 283–298 | 65–55 | FAM | KP331495 |
| R: GGTAACCTCATTGGGACCCG | ||||||
| PW65 | F: *CACAACAAGCGTTAATTTACGAGC | (ATC)33 | 359–368 | 65–55 | HEX | KP331496 |
| R: GTCATGCTCCTGCTCAGTCC | ||||||
| PW69 | F: *GGATGGTTTCTTCATGCAACG | (AAAAG)25 | 302–312 | 65–55 | HEX | KP331497 |
| R: AGCAAATCCTTGATCAGCCC | ||||||
| PW79 | F: *TTGCCAATAGTAACTCTTTGAACTCC | (ATGTCC)36 | 387–399 | 65–55 | FAM | KP331498 |
| R: CCAGAATGTGGGATTGAGGG | ||||||
| PW106 | F: CTAATATGAGCTTTAGCAACACTGC | (ATT)39 | 258–288 | 65–55 | FAM | KP331499 |
| R: *ATTATTCAACATGACACCATTAACC | ||||||
| PW182 | F: *TTGGTGACGACATTAGGAGGC | (AAAGG)30 | 308–318 | 60–50 | FAM | KP331500 |
| R: TAAGGGCAGCCGACCTAAGC | ||||||
| PW223 | F: *CGTAACACAACCTTACAATCTAAGCC | (ATC)36 | 438–447 | 65–55 | HEX | KP331501 |
| R: TGCTTTGTGAAGATCAGTGGG |
Note: Ta = range of annealing temperatures used in touchdown PCR.
*
Indicates the CAG sequence position (5′-CAGTCGGGCGTCATCA-3′).
a
Allele size is the range of observed alleles (including the CAG sequence length).
b
Fluorescent dye used for fragment analysis.