Figure 2.

(A) The HT1080 cells were treated with Huaier at a concentration of 0, 4 or 8 mg/ml for 24 or 48 h. Flow cytometry with Annexin-V/PI was used to quantify Huaier-induced apoptosis in the HT1080 cells. (B) Flow cytometry revealed an increase in the proportion of apoptotic cells following treatment with increasing concentrations of Huaier. (C) HT1080 cells were treated with 4 or 8 mg/ml Huaier for 48 h. The cell cycle distribution of the HT1080 cells was assessed using flow cytometry following staining with PI. (D) Huaier significantly inhibited the proliferation of HT1080 cells in a dose-dependent manner via cell-cycle arrest at the G₂ phase. ^**P<0.01 vs. control. FITC, fluorescein isothiocyanate; PI, propidium iodide; Ctrl, control; A, area.