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. 2015 Jan 27;9(4):1590–1596. doi: 10.3892/ol.2015.2906

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Cell mobility was strongly inhibited by Huaier extract in invasion and migration assays. (A) The invasive ability of the HT1080 cells following 17 h of treatment with Huaier was detected using the Transwell system. The cells were stained using the crystal violet staining agent. (B) The number of successfully invading HT1080 cells in the 4 mg/ml Huaier-treated group was significantly lower compared with the control group. (C) The migration ability of the HT1080 cells following 12 h of treatment with Huaier was detected using the Transwell system. The cells were stained using the crystal violet staining agent. (D) The number of successfully migrating HT1080 cells in the 4 mg/ml Huaier-treated group was significantly lower compared with the control group. (E) Images of the HT1080 cells treated with or without Huaier were captured at 0, 12 or 24 h during the scratch assay. (F) The distance between the edges of the scratch in the Huaier-treated group was greater than that of the control group. ^*P<0.05 vs. control, ^**P<0.01 vs. control.