Figure 2.

Loss of JUNB expression induces invasive prostate cancer. (a) IHC for JUNB in normal mouse dorsolateral prostate (wild type) and high-grade PIN (Pten^ΔP: Pten^flox/flox; PsaCre^+/T). Black arrows mark JUNB-positive cells in the epithelia. n≥3. A representative sample is shown. (b) Haematoxylin and eosin (H&E) staining of dorsolateral prostate from wild-type, JunB^ΔP (JunB^flox/flox; PsaCre^+/T) mice; n≥3. A representative sample is shown. (c) H&E staining of dorsolateral prostate from Pten^ΔP (Pten^flox/flox; PsaCre^+/T) and JunB^ΔP; Pten^ΔP (Junb^flox/flox; Pten^flox/flox; PsaCre^+/T) mice. L (lumen), T (tumour), S (stroma) and VP (ventral prostate). n≥3. A representative sample is shown. (d) Incidence of invasive prostate cancer in 12-week-old mice. n= Pten^ΔP: 0 out of 12; JunB^ΔP; Pten^ΔP: 6 out of 13. (e) Staining for basal cells with an antibody to p63 (brown). A representative noninvasive sample and an invasive sample are shown; n=5. (f) IHC for JUNB in noninvasive and invasive prostate cancer from JunB^ΔP; Pten^ΔP (Junb^flox/flox; Pten^flox/flox; PsaCre^+/T) mice. Black arrowheads mark JUNB-positive cells in the stroma. n≥3. A representative sample is shown. (g) Western blot for phospho-JNK in prostate samples from Pten^ΔP and JunB^ΔP; Pten^ΔP mice. Total JNK is used as loading control; n=3