Figure 4.
JUNB represses S100A8/9 and SPP1 in a non-cell-autonomous manner. (a) Western blots for S100A8 and S100A9 on prostate samples from PtenΔP and JunBΔP; PtenΔP mice. Vinculin was used as a loading control; n=3. (b) IHC for S100A9 in high-grade PIN and invasive dorsolateral prostate cancer from PtenΔP and JunBΔP; PtenΔP mice as indicated. n≥3. A representative sample is shown. (c) Quantitative reverse transcription PCR (qRT-PCR) for S100A8 and S100A9 in prostate samples from PtenΔP and JunBΔP; PtenΔP mice. n≥3. (d) qRT-PCR for Spp1 in prostate samples from PtenΔP and JunBΔP; PtenΔP mice. n≥3. (e) IHC for SPP1 in high-grade PIN and invasive prostate cancer from PtenΔP and JunBΔP; PtenΔP mice as indicated. n≥3. A representative sample is shown. (f) Box-plot diagram for S100A8, S100A9 and SPP1 mRNA expressions and correlation with JUNB expression from Chandran et al.14 (64 tumour samples and 24 metastasis samples) bioset