Table 2. Trypsin automated optimization trials.

Table detailing the ability of trypsin-MIPs and haemoglobin-MIPs to induce the nucleation of trypsin crystals under metastable conditions. Each trial was repeated eight times, with the number indicating the number of repeats where the nucleating agent facilitated crystal formation whilst the corresponding controls remained clear. At higher supersaturation both MIPs suffice, whilst at lower supersaturation (deeper in the metastable zone) the cognate MIP was more potent and reliable.

	PEG concentration
MIP sample	10%	11%	12%	13%	14%	15%
Trypsin-MIP	0	2	6	8	8	8
Haemoglobin-MIP	0	0	1	4	7	8