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. 2015 Feb 10;9(4):1839–1844. doi: 10.3892/ol.2015.2952

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Characteristics of cell lines and control fibroblast cultures and astrocytes, and reaction of different cell lines to co-culturing with glioma cells in the experiment. (A) Rat fibroblasts (hematoxylin and eosin; magnification, ×40). (B) Rat fibroblasts. Phagocytosis of collagen immobilized on the surface of the fluorescent microparticles FluoSpheres^® Collagen I-Labeled Microspheres (magnification, ×630). (C) Rat astrocytes stained with anti-GFAP Mab and nuclei counterstained with DAPI (magnification, ×100). (D) Rat astrocyte stained with anti-GFAP Mab (magnification, ×200). (E) Formation of fluorescent cell shaft on the perimeter of culture inserts containing glioma cells, by hematopoietic stem cells stained with Vybrant^® CFDA SE Cell Tracer (magnification, ×10). (F) Co-culturing hematopoietic stem cells and rat fibroblasts. The formation of the cell shaft was absent (magnification, ×10). GFAP, glial fibrillary acidic protein; Mab, monoclonal antibody.