Figure 1.

Conditioned medium (CM) secreted by PCa cells overexpressing OPNc (OPNc-CM) significantly activates the expression of AR responsive genes in LNCaP cells. Graphs showing relative RNA quantification of (A) Fgf8, (B) Tmprss2, (C) Greb1, (D) Cdk2, (E) Ndrg1, (F) Cdk1, (G) Pmepa1, (H) Ar and (I) Psa, in LNCaP cells cultivated with OPNc-CM compared with cells cultivated with the empty-vector CM (EV-CM), as described in the Materials and methods section. In order to test OPNc-specific effects, anti-OPNc polyclonal neutralizing antibody (α-OPNc), flutamide (FL), bicalutamide (BL) and PI3K inhibitor (LY), were used. 18S RNA was used as a constitutive gene in all these assays. Data are represented as the mean ± standard deviation. All experiments were biological replicates repeated a minimum of three times. ^*P<0.0001 vs. OPNc-CM cultivated cells. ^**P<0.0001 vs. EV-CM cultivated cells.