Figure 3.

Effects of WWOX on the differentiating capabilities of ovarian cancer stem cells. When plated in a serum-containing medium to allow for cellular differentiation, WWOX-expressing cells began to attach to the culture plate surface after 2 h and almost all cells had adhered to the culture plates. Untransfected cells and empty vector-transfected cells, by contrast, displayed a delay in attachment to the culture plate surface: A number of cells adhered to the culture plates after 6 h, whilst others maintained a spherical morphology and did not become adherent until 24 h. (A) Untransfected cells (6 h); (B) empty vector-transfected cells (6 h); (C) WWOX-expressing cells (2 h). Magnification, ×40. WWOX, WW domain-containing oxidoreductase.