Figure 6.

(A) Inhibition of intracellular lipid accumulation, (B) attenuation of scavenger receptor (SR)-B1 protein induction and (C) its transcription in 1–10 μM purple Perilla frutescens extracts (PPE)-α-asarone-treated and 50 μg/ml Cu²⁺-oxidized low-density lipoproteins (LDL)-exposed J774A.1 murine macrophages. (A) Foam cell formation was measured by staining the macrophages with Oil Red O. Microphotographs were obtained using an optical microscope. Magnification, x200. (B) For the measurement of SR-B1 expression, total cell lysates were subjected to western blot analysis with a primary antibody against SR-B1. β-actin was used as an internal control. Bar graphs (n=3) represent quantitative densitometric results of the upper bands. Bar graphs (means ± SEM) denoted without a common letter indicate significant difference, P<0.05. (C) SR-B1 mRNA expression was measured by RT-PCR. GAPDH was used as a housekeeping gene for the co-amplification with SR-B1.