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Effect of treatment with Pyropia yezoensis peptide [PYP1 (1–20)] on the levels of cell cycle-related proteins in IEC-6 cells. Cells were treated with PYP1 (1–20) after pre-incubation with SFM for 4 h. Whole cell extracts were prepared and analyzed by western blot analysis using (A) anti-cyclin D1, anti-cyclin E, anti-Cdk2, anti-Cdk4, anti-Cdk6 and anti-pRb; and (B) anti-p21, anti-p27 and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies.
