Table 4.
Statistical characteristics of 16S rRNA gene clone libraries derived from rice shoot-associated bacteria under various environmental conditions
| Tissues | Leaf blade | Leaf sheath | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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| Growth air conditions | Ambienta | FACEb | Ambient | FACE | ||||||||
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| Plots | ATc | ETd | LNe | AT | ET | LN | AT | ET | LN | AT | ET | LN |
| Statistics | ||||||||||||
| No. of sequences | 146 | 162 | 124 | 151 | 151 | 126 | 164 | 174 | 180 | 168 | 178 | 181 |
| No. of OTUs (≥97% identity)f | 47 | 50 | 44 | 46 | 49 | 32 | 77 | 76 | 78 | 81 | 82 | 82 |
| No. of singletons | 29 | 34 | 30 | 32 | 32 | 19 | 52 | 50 | 50 | 55 | 58 | 54 |
| Library coverage (%)g | 80.1 | 79 | 75.8 | 78.8 | 78.8 | 84.9 | 68.3 | 71.3 | 72.2 | 67.3 | 67.4 | 70.2 |
| Diversity indexes | ||||||||||||
| Chao1 | 114.7 | 130.1 | 98.4 | 128.7 | 119.9 | 74.8 | 209.6 | 187.4 | 180.0 | 216.0 | 288.6 | 241.0 |
| ACE | 148.9 | 179.5 | 105.6 | 290.7 | 224.8 | 60.5 | 332.3 | 289.3 | 367.6 | 397.8 | 398.7 | 280.1 |
| Shannon index (H′) | 3.0 | 2.7 | 2.9 | 2.7 | 3.0 | 2.2 | 3.9 | 3.8 | 3.9 | 4.0 | 3.9 | 4.0 |
| Simpson index (1/D) | 9.1 | 5.2 | 9.6 | 5.9 | 8.1 | 3.7 | 40.5 | 33.1 | 39.3 | 46.1 | 36.2 | 37.5 |
a
Rice plants grown under ambient atmosphere condition.
b
Rice plants grown under the free-air CO2 enrichment condition.
c
Rice plants grown in ambientl surface water-soil temperature with standard nitrogen fertilization.
d
Rice plants grown in elevated surface water-soil temperature (2°C above AT) with standard nitrogen fertilization.
e
Rice plants grown in ambient surface water-soil temperature with no nitrogen fertilization.
f
OTUs were defined at ≥97% sequence identity.
g
Coverage (Cx) = 1 − (nx/N), where nx is the number of singletons that are encountered only once in a library and N is the total number of clones.