Table 5.

Phylogenetic compositions of 16S rRNA gene clone libraries derived from leaf blade- and leaf sheath-associated bacteria in rice plants cultivated under different environmental conditions

	Relative abundance in a clone library (%)a
Tissues	Leaf blade						Leaf sheath
Atmospheric conditions	AMBIb			FACEc			AMBI			FACE
Temperature	NTd	ETe	LNf	NT	ET	LN	NT	ET	LN	NT	ET	LN
Proteobacteria	74.0	71.0	57.3**	88.7**	77.5	76.2	51.8	58.0	61.7	51.2	59.0	59.7
Alphaproteobacteria	30.8	19.1*	24.2	33.1	31.1	15.9**	29.9	29.9	32.8	29.8	29.2	33.7
Betaproteobacteria	3.4	5.6	—	8.6	7.9	1.6	4.9	9.2	5.0	7.7	10.7*	3.3
Gammaproteobacteria	37.7	46.3	32.3	45.0	37.1	58.7**	14.0	13.8	22.2	11.3	15.2	18.2
Deltaproteobacteria	2.1	—	0.8	—	—	—	0.6	3.4	1.7	2.4	2.8	1.1
Planctomycetes	1.4	3.7	—	0.7	1.4	0.8	30.5	27.6	20.0*	25.6	22.5	22.6
Firmicutes	17.1	19.2	36.3**	7.9*	13.9	15.1	4.2	1.2	2.8	5.4	6.1	3.6
Bacilli	11.6	15.5	27.4**	6.0	8.6	13.5	1.8	0.6	1.7	3.0	3.3	1.7
Clostridia	5.5	3.7	8.9	2.0	5.3	1.6	2.4	0.6	1.1	2.4	2.8	2.2
Actinobacteria	2.7	0.6	3.2	2.0	4.0	0.8	0.6	1.7	1.1	1.8	—	1.7
Verrucomicrobia	0.7	0.6	0.8	—	—	—	1.8	2.9	2.2	3.0	2.2	1.7
Acidobacteria	—	—	—	—	—	1.6	1.2	0.6	1.7	1.8	—	0.6
Cyanobacteria/Chloroplast	—	—	—	—	0.7	—	0.6	—	—	—	—	—
Chloroflexi	—	—	0.8	0.7	—	—	—	—	—	—	—	—
BRC1	—	—	—	—	—	—	—	—	—	0.6	—	—
Unclassified bacteria	4.1	4.9	1.6	—	2.6	5.6	9.1	8.0	10.6	10.7	10.1	8.3

^{a* and **}indicate statistical significance at the 1 and 5% levels (P < 0.01 and P < 0.05), respectively, calculated with the Library Compare of RDP II, between the control (AMBI-NT) and other samples.

^bRice plants grown under ambient atmosphere condition.

^cRice plants grown under the free-air CO₂ enrichment condition.

^dRice plants grown in normal surface water-soil temperature with standard nitrogen fertilization.

^eRice plants grown in elevated surface water-soil temperature (2°C above NT) with standard nitrogen fertilization.

^fRice plants grown in normal surface water-soil temerature with low nitrogen fertilization.