Figure 1.

Effects of 5-FU on the growth of (A) oral cancer (B88) cells and (B) oral keratinocytes (RT7). The cells were grown in 96-well plates in medium supplemented with 5-FU [0 (⋄), 1 (●), 2 (△), 5 (▲) and 10 (□) μg/ml] for 3 days. Viable cells were estimated by 3-(4,5-dimethylthi-azol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance was measured at 570 nm. Standard deviations were calculated from three independent experiments. Growth was significantly lower compared to that in untreated cells. Although B88 cells were decreased in number by treatment with 5 and 10 μg/ml 5-FU, there was no apparent decrease in cell number when RT7 cells were treated with the same concentrations of 5-FU. Statistically significant at ^*P<0.05 and ^**P<0.01 (Mann-Whitney U test).