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. 2015 Jan 26;46(4):1453–1460. doi: 10.3892/ijo.2015.2849

Figure 2.

Figure 2

Effects of reactive oxygen species (ROS) on the growth of RT7 cells. (A) Generation of ROS in 5-FU-treated RT7 cells. The levels of ROS production were measured at 24 and 48 h after treatment of RT7 cells with 5-FU (10 μg/ml) using the ROS detection dye DCFH-DA. The total amount of ROS produced was significantly augmented at 48 h after treatment. (B) Effect of N-acetyl cysteine (NAC) on the generation of 5-FU-induced ROS. NAC (0.1 mM), a ROS scavenger, significantly suppressed the production of ROS at 48 h after treatment with 5-FU (10 μg/ml). (C) NAC improvement of cell growth in 5-FU-treated RT7 cells. Cells were treated with 5-FU (△), NAC (●), or both (▲). Combined treatment of RT7 cells with 5-FU (10 μg/ml) and NAC (0.1 mM) significantly recovered the impairment of cell growth as compared to the treatment with 5-FU alone. Statistically significant at *P<0.05 (Mann-Whitney U test).