Figure 3.

Effects of γ-tocotrienol on the growth and production of reactive oxygen species (ROS) in RT7 cells. (A) Cells were grown for 3 days in 96-well plates containing medium supplemented with 5-FU (10 μg/ml, △), γ-tocotrienol (γT3, 10 nM, ●), or both (▲). γ-tocotrienol treatment significantly restored the 5-FU-induced growth inhibition in RT7 cells. (B) Effect of γ-tocotrienol on the generation of 5-FU-induced ROS. γ-tocotrienol (γT3, 10 nM) significantly suppressed the production of ROS at 48 h after treatment with 5-FU (10 μg/ml). Statistically significant at ^*P<0.05 and ^**P<0.01 (Mann-Whitney U test).