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. 2015 Mar 11;10(3):e0119577. doi: 10.1371/journal.pone.0119577

Fig 3. p47phox enables Angpt-1 to counteract cellular structural rearrangements induced by thrombin.

Fig 3

(A-H) Confluent HMVECs were treated with control siRNA, thrombin (1 U/ml, A-D), or the combination of Angpt-1 (300 ng/ml) plus thrombin (E-H). After 15 minutes, cells were fixed, permeabilized, and stained for nuclei (blue, DAPI), VE-cadherin (green), or F-actin (red). White arrows indicate paracellular gaps. (I-P) Repeat of the above experiments, replacing control siRNA with p47phox siRNA. Representative of n = 3–4 experiments per condition. Scale bar 10 μm.