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. 2015 Mar 11;10(3):e0119639. doi: 10.1371/journal.pone.0119639

Fig 2. Identification of NIb as the HR-inducing avirulence factor against Pvr4-bearing pepper plants.

Fig 2

(A) Transient expression of PepMoV viral proteins in CM334 and Jupiter. Eleven cistrons from PepMoV were infiltrated into CM334 and Jupiter. At 3 dpi, leaves were cleared with 100% ethanol to remove chlorophylls in order to visualize the cell death. For this and subsequent experiments, Empty vector and vector with necrosis-inducing protein (NIP) from P. sojae were infiltrated as a negative or positive control, respectively. Regions of infiltration were marked with ovals and the area of cell death was marked as red. Inoculated viral cistrons were depicted under panels. (B) Transient expression of HC-Pro:HA, CP:HA and NIb:HA in CM334. Plant responses with HA-tagged proteins were tested in Pvr4-harboring plants (CM334). Inoculated viral cistrons were depicted under panels. (C) Expression of PepMoV NIb:HA, CP:HA and HC-Pro:HA proteins in N. benthamiana leaves. 5-week-old tobacco leaves were collected at 24hpi and 48hpi. Untreated leaves were used as mock for negative controls. Each protein was immunodetected by using anti-HA antibody. Coomassie blue–stained total proteins were shown as loading controls.