Table 1. Concentrations and vehicles used for each reference compound during assay development.

Compound	Abbreviation	Vehicle	Max solubility (μM)	Rv90 (μM)	GARD input concentratrion (μM)
Respiratory sensitizers
Ammonium hexachloroplatinate	AH	Water	35	-	35
Ammonium persulfate	AP	DMSO	-	-	500
Ethylenediamine	EDA	Water	-	-	500
Glutaraldehyde	GA	Water	-	10	10
Hexamethylen diisocyanate	HDI	DMSO	100	-	100
Maleic Anhydride	MA	DMSO	-	-	500
Methylene diphenol diisocyanate	MDI	DMSO	50	-	50
Phtalic Anhydride	PA	DMSO	200	-	200
Toluendiisocyanate	TDI	DMSO	40	-	40
Trimellitic anhydride	TMA	DMSO	150	-	150
Non-Respiratory sensitizers
1-Butanol	BUT	DMSO	-	-	500
2-Aminophenol	2AP	DMSO	-	100	100
2-Hydroxyethyl acrylate	2HA	Water	-	100	100
2-nitro-1,4-Phenylenediamine	NPDA	DMSO	-	300	300
4-Aminobenzoic acid	PABA	DMSO	-	-	500
Chlorobenzene	CB	DMSO	98	-	98
Dimethyl formamide	DF	Water	-	-	500
Ethyl vanillin	EV	DMSO	-	-	500
Formaldehyde	FA	Water	-	80	80
Geraniol	GER	DMSO	-	-	500
Hexylcinnamic aldehyde	HCA	DMSO	32.34	-	32.34
Isopropanol	IP	Water	-	-	500
Kathon CG*	KCG	Water	-	0.0035%	0.0035%
Methyl salicylate	MS	DMSO	-	-	500
Penicillin G	PEN G	Water	-	-	500
Propylene glycol	PG	Water	-	-	500
Potassium Dichromate	PD	Water	51.02	1.5	1.5
Potassium permanganate	PP	Water	38	-	38
Tween 80	T80	DMSO	-	-	500
Zinc sulphate	ZS	Water	126	-	126

^*The chemical Kathon CG is a mixture of the two compounds MC and MCI. The concentration of the mixture is given in %.