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. 2015 Mar 11;11(3):e1004723. doi: 10.1371/journal.ppat.1004723

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© 2015 Carpentier et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) SDS-PAGE and immunoblot analysis of α-HA-IP from HEK 293T cells infected with either vF12-HA or vB14-HA at 5 PFU/cell and harvested 14 hpi. Clarified cell lysates (Input) and α-HA immunoprecipitated samples were immunoblotted with the antibodies indicated on the left of the figure. (B) SDS-PAGE and immunoblot analysis of α-HA immunoprecipitation from lysates generated from HEK 293T cells transfected with a plasmid encoding HA-tagged E2 or a control plasmid as indicated. Samples were probed for the precipitated E2 protein (i) and for co-precipitation of KLC using the 63–90 antibody (ii). The co-precipitating KLC isoform identity was confirmed by immunoblotting with antibodies specific for KLC1 (iii) and KLC2 (iv). Co-precipitation of the entire kinesin-1 complex with E2 was confirmed by immunoblotting with the α-Kif5B antibody (v).