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. 2014 Oct 25;(92):51721. doi: 10.3791/51721
20 nM INO80 or INO80 subcomplexes
2.8 nM nucleosomes (consisting of a mixture of mononucleosomes on the 32P-labeled 601 DNA fragment and Hela cell nucleosomes)
1 mM ultrapure ATP
20 mM HEPES-NaOH (pH 7.9)
50 mM NaCl
5 mM MgCl2
1 mM dithiothreitol (DTT)
0.1 mM phenylmethanesulfonyl fluoride (PMSF)
0.1 mg/ml bovine serum albumin (BSA)
5% glycerol
0.02% Nonidet P-40
0.02% Triton X-100