10 mM | HEPES pH 7.9 |
10% | glycerol |
100 mM | NaCl |
1.5 mM | MgCl2 |
0.05% | TritonX-100 |
Add just before use: | |
1 mM | DTT |
200 µM | PMSF |
1:1,000 dilution | Protease Inhibitor Cocktail (see Materials List) |
10 mM | HEPES pH 7.9 |
10% | glycerol |
100 mM | NaCl |
1.5 mM | MgCl2 |
0.05% | TritonX-100 |
Add just before use: | |
1 mM | DTT |
200 µM | PMSF |
1:1,000 dilution | Protease Inhibitor Cocktail (see Materials List) |