Fig 3. CD271-based enrichment of MSCs from RIA waste fluid.
Inter-operative images of the RIA device showing the main body of the device (left panel), the reamer head passing through the intramedullary canal (middle panel) and the waste fluid collection bag (right panel) (A). The proportions of CD45−/lowCD73+ (B) and CD45−/lowCD73+CD271+ cells (C) in RIA waste fluid pre and post CliniMACS enrichment, with representative dot plots shown below. The proportion of CFU-Fs pre and post CliniMACS enrichment and representative CFU-F dishes below (D). The proportion of CD45+CD271low ‘passenger’ cells (E), CD45+ leukocytes (F) and total live cells (G) pre and post CliniMACS enrichment. (All data n = 3).