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. 2015 Mar 11;10(3):e0117855. doi: 10.1371/journal.pone.0117855

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© 2015 Cuthbert et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Differentiation potential of PA-MSCs and CD271-MSCs. Osteogenesis, adipogenesis and chondrogenesis was measured on day-21 post-induction by staining with alkaline phosphatase/von Kossa, Oil Red O and Alcian Blue, respectively in cells selected from BM (A), FH (B) and RIA (C), all size bars represent 500μm. Phenotypic analysis of the CD45^−/lowCD271⁺ population observed in BM (D), FH (E) and RIA (F). Analysis of the total expression of BMP-2, COL1A2, VEGFC, SPARC and CXCL12 transcripts relative to HPRT in BM (G), FH (H) and RIA (I) pre (black bars) and post (grey bars) CD271 enrichment. (All data n = 3).