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. 2015 Mar 11;11(3):e1005051. doi: 10.1371/journal.pgen.1005051

Fig 6. Cooperative regulation between the cluster and surrounding motifs produces robust class-specific expression.

Fig 6

(A) GFP expression (green) driven by the 2×Or59b cluster at 14°C and 24°C. Note the three expression phenotypes produced by the 2×Or59b cluster at 14°C. Synaptic neuropil regions are labeled with the presynaptic marker nc82 (magenta). (B) GFP expression (green) produced by the Or85a cluster at 14°C, 24°C or following 3 days of starvation. Note that GFP expression is equally strong in different lines at 14°C. (C) The Or59b cluster with an additional E-box produces robust expression at 14°C and 24°C. (D) The fractions of the brains showing stable or bimodal expression of GFP according to the genotype and temperature. Note that only the Or59b cluster and 2×Or59b cluster are unstable at 14°C. (E) Quantification of GFP positive cells in the antenna. Or59b cluster shows a varied number of GFP positive cells at 14°C compared to 24°C. Or59b and Or59b cluster with an additional E-box show stable expression in both temperatures. Schematic interpretations of the results are presented in S6 Fig.