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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Pathol. 2015 Jan 7;235(5):731–744. doi: 10.1002/path.4484

Fig 1. TrkB expression in renal tissue and cultured podocytes.

Fig 1

A) RT-PCR results show presence of the full-length (281 bp) and truncated (353 bp) TrkB isoforms in preparations from brain (lane 1), cerebellum (lane 2), and isolated mouse glomeruli (lane 3 and 4). MWM: molecular weight marker; lane 5: negative control, i.e. PCR products obtained from the osteoblast cell line MC3T3-E1; lane 7: PCR negative control.

B) Western Blot (WB) analysis was conducted on protein extracts from mouse brain (1), cerebellum (2) and two preparations of isolated glomeruli (3, 4) in non-reducing conditions. A band slightly higher than 120kDa, corresponding to the full length molecule, is present in all lanes. In addition, higher bands are observable. Bands of lower molecular weight can be detected as well. MWM = molecular weight marker, Lane 5 = negative control, performed by loading protein lysates from the osteoblast cell line.

C) Immunogold, conducted on a cryosection from control mouse kidney, shows TrkB positivity in a podocyte (red circles) and a dot in an endothelial cell (light blue circle). Scale bar 500nm.

D) Double staining shows that TrkB mostly co-localizes with nephrin (upper panels) and seldom with CD31 (lower panels, arrow). Scale bars 10μm.