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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Pathol. 2015 Jan 7;235(5):731–744. doi: 10.1002/path.4484

Figure 6. In vivo glomerular TrkB silencing impairs BDNF effects.

Figure 6

Representative light microscopy (upper panels, Hematoxilin-Eosin, scale bars 50 μm) shows a damaged glomerulus surrounded by dilated tubuli filled by protein casts in a mouse treated with saline (left panel). BDNF is effective in reducing renal damage in a mouse injected with scramble siRNA (middle panel), whereas the efficacy is blunted after silencing of TrkB (right panel).

Nephrin positivity (second row) can be clearly observed in a BDNF/scramble injected animal (middle panel), whereas is barely detectable in glomeruli from a saline-treated and a BDNF/TrkB-silenced mouse.

Cofilin staining (third row) does not show major changes in all experimental conditions. Instead, P-cofilin (fourth row) is almost negative after saline (left panel) and BDNF/TrkB silencing (right panel), and is clearly positive after BDNF/scramble siRNA (middle panel). Scale bars 25 μm.