FIG 2.

M. smegmatis is susceptible to PZP in an iron-dependent, ESX-3-independent manner. (A) Growth of M. smegmatis WT and ΔfxbA was monitored by OD₆₀₀ measurements for 80 h under iron-replete (right) and iron-deplete (left) conditions, in the presence or absence of 25 μM PZP. M. smegmatis Δesx-3 ΔfxbA was included as a control. Results are representative of two independent experiments. (B) Growth of M. smegmatis WT and Δesx-3 was monitored for 70 h in iron-replete (right) and iron-deplete (left) medium, in the presence or absence of 25 μM PZP. (C) Growth of M. smegmatis WT (left) and Δesx-3 (right) was monitored for 70 h in iron-deplete medium in the presence of increasing concentrations of PZP. Results shown in panels B and C represent the averages of three independent biological replicates.