TABLE 3.
Effect of proline on drug susceptibility in L. donovania
| Line | EC50 μM (RI)b |
||
|---|---|---|---|
| AmB | MIL | SbIII | |
| WTM | 0.063 ± 0.002 | 3.45 ± 0.19 | 96.94 ± 8.91 |
| WTM + proline | 0.060 ± 0.002 (0.95 ± 0.07) | 3.24 ± 0.06 (0.94 ± 0.07) | 191.96 ± 8.62 (1.98 ± 0.14)c |
a
Promastigote forms of WTM were grown with or without 200 mM proline and incubated for 120 h at 28°C in the presence of increasing concentrations of oxidative stress-inducing drugs, as described in Materials and Methods. Cell viability was determined using an MTT-based assay.
b
The resistance index (RI) was calculated by dividing the EC50 for WTM grown with proline by the EC50 for WTM grown without proline, which is shown in parentheses. The data are the means of EC50 or RI ± SD from three independent experiments.
c
Significant differences were determined using Student's t test. The result of 191.96 ± 8.62 was significant at a P value of <0.001.