TABLE 5.

Membrane fluidity and drug accumulation in L. donovani WTM and resistant lines^a

Line	Anisotropy values	% drug accumulationb
		AmB	MIL	PMM	SbIII
WTM	0.220 ± 0.010	100 ± 7.23	100 ± 8.43	100 ± 7.43	100 ± 1.06
A	0.290 ± 0.001**	100.28 ± 0.69
M	0.230 ± 0.028		101.01 ± 4.42
P	0.251 ± 0.001*			86.05 ± 7.80
S	0.244 ± 0.003*				109.48 ± 11.52
AS	0.246 ± 0.003*	100.25 ± 0.54			233.23 ± 11.79***
SP	0.266 ± 0.001*			91.47 ± 9.48	145.42 ± 8.22***

^aSteady-state fluorescence anisotropy of DPH was incorporated in plasma membranes of different Leishmania lines. The drug accumulation assay was performed after incubating Leishmania lines with 0.1 μM AmB, 2.5 μM MIL, 10 μM PMM, or 100 μM Sb^III, as described in Materials and Methods. Significant differences versus WTM line were determined by Student's t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).

^bThe data are the means ± SD from three independent experiments.