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. 2015 Mar 11;59(4):2242–2255. doi: 10.1128/AAC.04231-14

TABLE 5.

Membrane fluidity and drug accumulation in L. donovani WTM and resistant linesa

Line Anisotropy values % drug accumulationb
AmB MIL PMM SbIII
WTM 0.220 ± 0.010 100 ± 7.23 100 ± 8.43 100 ± 7.43 100 ± 1.06
A 0.290 ± 0.001** 100.28 ± 0.69
M 0.230 ± 0.028 101.01 ± 4.42
P 0.251 ± 0.001* 86.05 ± 7.80
S 0.244 ± 0.003* 109.48 ± 11.52
AS 0.246 ± 0.003* 100.25 ± 0.54 233.23 ± 11.79***
SP 0.266 ± 0.001* 91.47 ± 9.48 145.42 ± 8.22***
a

Steady-state fluorescence anisotropy of DPH was incorporated in plasma membranes of different Leishmania lines. The drug accumulation assay was performed after incubating Leishmania lines with 0.1 μM AmB, 2.5 μM MIL, 10 μM PMM, or 100 μM SbIII, as described in Materials and Methods. Significant differences versus WTM line were determined by Student's t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).

b

The data are the means ± SD from three independent experiments.