TABLE 3.
Characterization of recombinant neuraminidases in the NI fluorescence assay
| NA change | Mean IC50 ± SD,a nM (fold change)b |
Avg NA activity, % (fold change)c | |||
|---|---|---|---|---|---|
| Zanamivir | Oseltamivir | Peramivir | Laninamivir | ||
| del245-248 | 3.48 ± 0.48 (21) | 22.29 ± 1.43 (222) | 0.35 ± 0.04 (5) | 0.42 ± 0.06 (1) | 68 (2) |
| del247-250 | 2.74 ± 0.31 (17) | 23.56 ± 3.92 (235) | 0.41 ± 0.02 (5) | 0.40 ± 0.07 (1) | 65 (2) |
| A/Perth/16/2009 | 0.16 ± 0.05 | 0.10 ± 0.04 (1) | 0.07 ± 0.03 (1) | 0.23 ± 0.02 (1) | 100 (1) |
a
Data are based on the results of at least three independent experiments.
b
Fold increase based on comparison to the respective IC50s of the recombinant NA with no deletion (wild type).
c
Fold decrease based on comparison to the NA activity of recombinant NA with no deletion (wild type).