FIG 4.
Effects of QC on farnesol production. Supernatant was collected from QC-treated or untreated broth culture of NBC099 cells and extracted thrice with a one-fifth volume of ethyl acetate. The solvent was removed, and the residue was resuspended in absolute ethanol and applied to silica gel 60G F254 plates. Separation of farnesol was obtained with a solvent system consisting of toluene-ethyl acetate-formic acid (7.5:2:0.5), and the results were quantified by HPTLC with CAMAG winCATS IV software. Panels: A, UV at 254 nm; B, overlapping spectra; C, quantified contents of farnesol versus concentrations of QC (inset, incubation times). Each value is the mean result ± the standard deviation of two independent experiments.