TABLE 1.
Three mutations related to metronidazole resistance confirmed in 3 genes by PCR-based sequencing
| Genea | Position | Mutation type | Wild typeb | Mutationc | Mutation in straind: |
|
|---|---|---|---|---|---|---|
| 26695-1MET | 26695-1 | |||||
| frxA (hp0642) | 571 | Indel | TA | + | − | |
| rdxA (hp0954) | 3 | SNPe | G | A | + | − |
| rpsU (hp0562) | 37 | SNP | G | T | + | − |
a
frxA encodes a NAD(P)H-flavin oxidoreductase; rdxA encodes an oxygen-insensitive NAD(P)H nitroreductase; rpsU encodes the 30S ribosomal protein S21.
b
The wild type column shows the nucleotide or segment of nucleotide in the wild-type strain.
c
The mutation column shows the nucleotide mutation or insertion mutations found in the mutant strain.
d
Strain 26695-1MET is a metronidazole-resistant strain; 26695-1 is a wild-type strain (26695). +, nucleotide mutation or insertion mutations occurred; −, no nucleotide mutation or insertion mutations occurred.
e
SNP, single-nucleotide polymorphism.